p53, a nuclear protein, is believed to play an important role, through mutation and overexpression, in the progression of human malignant tumors. And decreased expression of nm23-H1 protein is also believed that it is associated with metastatic
potential of malignant tumor. Mouse monoclonal antibody NCL-p53-DO7 (Novocastra Laboratories Ltd., Newcastle, England) which specifically immunoprecipitate p53 protein, and mouse monoclonal antibody NCL-nm23-H1(Novocastra Laboratories Ltd.,
Newcastle,
England) were used to examine 30 paraffin-embedded gastric carcinoma to find nuclear expression of mutant-form p53 protein and intracellular expression of nm23-H1 protein.
@ES The results obtained are as follows:
@EN 1. Positivity of p53 protein is more frequent in advanced gastric carcinoma with lymph node metastasis than early gastric carcinoma (p<0.06). there is significant correlation between expression of p53 protein and clinicopathologic finding of
gastric
carcinoma. But there is no significant correlation between expression of nm23-H1 protein and clinicopathologic finding of gastric carcinoma.
2. There is no significant correlation between expression of p53 and nm23-H1 protein and histopathologic differentiation of gastric carcinoma.
3. There is significant correlation between positivity of p53 and invasion depth of carcinoma. Positive reaction is more frequent in carcinomas with serosa or omental invasion rather than carcinomas with mucosa, submucosa, or muscular invasion
(p<0.03).
4. Survival rat of p53 and nm23-H1 negative groups are better than positive group (p53:p<0.02, nm23-H1:p<0.05)
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